Advanced Course in Immunology On Demand Lecture Recordings-Innate Immunity: Gene Regulation

Video Transcription

Video Summary

The transcript is a lecture on gene regulation during the inflammatory response, with a focus on macrophages. It explains how innate immune cells rapidly detect microbes through germline-encoded receptors such as TLRs, RIG-I-like receptors, cGAS, NOD-like receptors, and c-type lectins. This triggers signaling cascades that activate transcription factors including NF-kappa-B, IRF3, and STATs, which turn on key inflammatory genes like TNF, IL-6, IL-1, chemokines, adhesion molecules, interferons, and IL-10.<br /><br />A major theme is timing: some genes are “early” and poised for rapid activation, while others are “late” and require new protein synthesis, chromatin remodeling, or specific co-factors. The talk covers regulation at multiple levels, including transcription, mRNA processing, splicing, mRNA stability, translation, epigenetic modification, enhancer activity, and chromatin looping. It highlights how promoters and enhancers, histone marks, paused polymerase, and complexes like SWI/SNF, mediator, and cohesin shape gene expression kinetics.<br /><br />The speaker also discusses emerging topics such as alternative splicing, long non-coding RNAs, condensates, CRISPR screening, and innate immune memory/trained immunity. The lecture emphasizes that inflammatory gene regulation is highly cell-type specific and still not fully understood, especially in macrophages.

Asset Subtitle

Lecture 4: Susan Carpenter, University of California, Santa Cruz

Innate Immunity: Gene Regulation

Asset Caption

Susan Carpenter, University of California, Santa Cruz, Innate Immunity: Gene Regulation

Keywords

gene regulation

inflammatory response

macrophages

innate immune receptors

TLRs

NF-kappa-B

IRF3

cytokines

chromatin remodeling

enhancers

alternative splicing

trained immunity

CRISPR screening