IMMUNOLOGY2026™ Conference Recordings For Attendees-Bridging the Gap in Flow Cytometry Training: A Practical Workflow for Teaching Autofluorescence Management in Immunology

Bridging the Gap in Flow Cytometry Training: A Practical Workflow for Teaching Autofluorescence Management in Immunology

Back to course

Video Transcription

Video Summary

The talk described a workflow for improving spectral flow cytometry analysis by reducing autofluorescence-related unmixing errors. The speaker explained that complex tissues often contain multiple autofluorescent signatures that interfere with reference controls and cause poor resolution, especially for dim markers. Their approach uses a non-stained sample to identify problematic autofluorescence peaks, then manually “cleans” matching signatures out of reference controls before unmixing. This makes the spectral references better reflect what the algorithm actually sees in real samples. The method was applied across many tissue types, including lung, spleen, blood, intestine, brain, and atherosclerotic vessels, and reportedly improved reproducibility, reduced analysis time, and helped with low-signal markers and activation assays. They also noted that compensation beads were less useful because their autofluorescence does not match cells well.

Meta Tag

Date April 16, 2026 11:00 AM - 11:15 AM

Room 104AB

Session Immunology Education and Communication

Speaker Alina Moriarty

Track Immunology Education and Communication (EDU)

Year 2026

Keywords

spectral flow cytometry

autofluorescence

unmixing errors

reference controls

dim markers

April 16, 2026 11:00 AM - 11:15 AM

104AB

Immunology Education and Communication

Alina Moriarty

Immunology Education and Communication (EDU)

2026