IMMUNOLOGY2026™ Conference Recordings For Attendees-Exportin-5 Links miRNA Biogenesis to the Preservation of B Cell Receptor Surface Expression

Exportin-5 Links miRNA Biogenesis to the Preservation of B Cell Receptor Surface Expression

Video Transcription

Video Summary

Tylar Kirch described research linking XPO5 and microRNA biogenesis to B-cell receptor (BCR) surface expression. A genome-wide CRISPRi screen in Ramos B cells identified XPO5 as a key regulator of BCR levels. XPO5 knockdown reduced surface BCR without changing BCR transcript levels or other surface markers, suggesting post-transcriptional control. Loss of XPO5 also impaired proliferation and accelerated AID-mediated BCR loss through increased harmful mutations, especially in the light chain. XPO5 depletion lowered mature microRNAs globally, altering hundreds of genes involved in cell cycle and DNA repair, which likely drives the observed phenotypes.

Meta Tag

Date April 16, 2026 10:45 AM - 11:00 AM

Room 153AB

Session Epigenetics and Post-Transcriptional Regulation

Speaker Tylar Kirch

Track Immune Response Regulation: Molecular Mechanisms (IRM)

Year 2026

Keywords

XPO5

microRNA biogenesis

B-cell receptor

CRISPRi screen

AID-mediated mutation

April 16, 2026 10:45 AM - 11:00 AM

153AB

Epigenetics and Post-Transcriptional Regulation

Tylar Kirch

Immune Response Regulation: Molecular Mechanisms (IRM)

2026