IMMUNOLOGY2026™ Conference Recordings For Attendees-Generation and Performance of a DNA Barcode-Based Nuclear Receptor Reporter Library

Generation and Performance of a DNA Barcode-Based Nuclear Receptor Reporter Library

Video Transcription

Video Summary

Hui-Juan from Dartmouth presented NR-Seq, a DNA barcode-based nuclear receptor reporter library designed to overcome limitations of traditional nuclear receptor assays. Nuclear receptors are important transcription factors involved in metabolism, immune regulation, and disease, and many still lack known ligands. Her system uses stable transduction of all-in-one reporter plasmids with dual barcodes, enabling both single-plex flow validation and multiplex bulk RNA-seq readouts. The library showed strong, specific, and stable responses over eight weeks. Future applications include screening bioassays, gut microbial metabolites, drug repurposing, and eventually in vivo reporter models in mice.

Meta Tag

Date April 18, 2026 1:00 PM - 1:15 PM

Room 151

Session Technical Innovations for Therapies

Speaker Huijuan Yang

Track Technological Innovations in Immunology (TECH)

Year 2026

Keywords

NR-Seq

nuclear receptor reporter library

DNA barcode

dual barcodes

multiplex RNA-seq

April 18, 2026 1:00 PM - 1:15 PM

151

Technical Innovations for Therapies

Huijuan Yang

Technological Innovations in Immunology (TECH)

2026