IMMUNOLOGY2026™ Conference Recordings For Attendees-High-throughput TCR-epitope screening using the Jurkat NFAT Reporter-K562 APC cell system on the Microwell ELISpot Platform

High-throughput TCR-epitope screening using the Jurkat NFAT Reporter-K562 APC cell system on the Microwell ELISpot Platform

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Video Summary

The speaker described a pipeline for screening peptide–T cell receptor interactions, mainly to support cancer vaccine and T cell therapy development. Their group developed three related tools: bulk TCR/BCR repertoire profiling with barcode-based error correction, single-cell sequencing for paired receptor chains and phenotype, and Jurkat/K562 artificial cell systems for testing peptide–TCR binding.<br /><br />They showed a workflow using MART1 peptide-stimulated PBMCs, where repertoire profiling identified expanded clonotypes after two weeks. These were validated by CD137, IFN-gamma, and machine-learning predictions, then cloned into Jurkat reporter cells, where most triggered strong GFP signals. They also used a micro-well imaging platform to co-culture dendritic cells and T cells, allowing real-time detection of IFN-gamma secretion and isolation of reactive clones with nanomolar sensitivity. Finally, they proposed scaling up by screening peptide libraries against TCR libraries, demonstrating a 120-peptide library screen that correctly identified CMV-specific responses.

Meta Tag

Date April 18, 2026 1:45 PM - 2:00 PM

Room 151

Session Technical Innovations for Therapies

Speaker Alex Chenchik

Track Technological Innovations in Immunology (TECH)

Year 2026

Keywords

peptide-TCR screening

T cell receptor repertoire

cancer vaccine development

single-cell sequencing

Jurkat reporter assay

April 18, 2026 1:45 PM - 2:00 PM

151

Technical Innovations for Therapies

Alex Chenchik

Technological Innovations in Immunology (TECH)

2026