IMMUNOLOGY2026™ Conference Recordings For Attendees-Multiomic profiling in Longitudinal Transplantation studies for Innate and Adaptive Immunity Insights

Multiomic profiling in Longitudinal Transplantation studies for Innate and Adaptive Immunity Insights

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Video Summary

The speaker described extensive multi-omics studies in brain-dead pig xenotransplant models, where frequent sampling of blood, urine, biopsies, and autopsy tissues enabled near-daily tracking of transplant biology. Over a 61-day heart/kidney case, they observed antibody-mediated and T-cell–mixed rejection, as well as early graft injury linked to pig macrophages and infections. The team combined single-cell RNA-seq, spatial transcriptomics (Xenium), and deep proteomics to map immune responses, complement activation, and pig-specific damage markers. They also analyzed pig liver xenografts supported on perfusion systems and used mixed-species spatial platforms to distinguish pig and human cell interactions. New work included spatial RNA/proteomics/metabolomics and single-nucleus methylated whole-genome atlases to interpret cell-free DNA, aiming to identify cell- and tissue-specific sources of injury and rejection.

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Date April 17, 2026 8:00 AM - 9:30 AM

Room 151

Session The Immunology of Xenotransplantation: From Bench Discovery to Clinical Translation, Sponsored by the Amer. Soc. of Transplantation (AST)

Speaker Brendan Keating

Track Transplantation Immunology (TRAN)

Year 2026

Keywords

xenotransplantation

multi-omics

rejection

spatial transcriptomics

pig xenograft

April 17, 2026 8:00 AM - 9:30 AM

151

The Immunology of Xenotransplantation: From Bench Discovery to Clinical Translation, Sponsored by the Amer. Soc. of Transplantation (AST)

Brendan Keating

Transplantation Immunology (TRAN)

2026