IMMUNOLOGY2026™ Conference Recordings For Attendees-Rapid Assembly of Oligo Pools into Medium-Scale T Cell Receptor Lentiviral Libraries

Rapid Assembly of Oligo Pools into Medium-Scale T Cell Receptor Lentiviral Libraries

Video Transcription

Video Summary

The speaker presented a PhD method for cheaply and quickly synthesizing and transducing pooled T cell receptor (TCR) libraries. The goal is to streamline functional validation of TCR hits from single-cell and tetramer-based antigen discovery, since many annotated TCRs are not truly antigen-specific. Their approach, called CREP, uses codon-optimized TRV genes and pooled CDR3 oligos assembled by PCR in plates, allowing specific pairing and reducing cost and time. In early tests, the method showed no dropouts in a 13-TCR library and later worked on a 123-TCR library at about $10 per TCR. Functional screening identified candidate epitope-specific TCRs, and the workflow can be done in a day with standard cloning tools. Future work aims to scale this to cohort-level antigen screening and broader repertoire studies.

Meta Tag

Date April 16, 2026 1:45 PM - 2:00 PM

Room 205

Session Innovations in Bench Techniques

Speaker Connor Devine

Track Technological Innovations in Immunology (TECH)

Year 2026

Keywords

TCR library synthesis

CREP workflow

CDR3 oligo assembly

functional TCR screening

antigen-specific TCR validation

April 16, 2026 1:45 PM - 2:00 PM

205

Innovations in Bench Techniques

Connor Devine

Technological Innovations in Immunology (TECH)

2026